BD Vacutainer PPT is a closed system allowing separation and storage of undiluted EDTA plasma in the primary blood collection tube. It is intended for the purposes of molecular diagnostic testing.

PPT is an evacuated, sterile blood collection tube that contains an inert gel and spray-dried K2EDTA anticoagulant for achieving plasma separation. When the sample-filled tube is subjected to centrifugation, the gel migrates and forms a physical barrier between the plasma and most of the cellular elements. Since the tube uses spray-dried K2EDTA as opposed to liquid additives, the plasma obtained is undiluted.

Preparation And Storage

Store unfilled tubes at 4-25ºC. Limited excursion temperatures up to 40ºC, for a cumulative time not to exceed 10 days, are acceptable.

Features

• Mylar label
• Volume: 5 mL
• Type: Plasma Tube
• Dimensions: 13 X 100 mm
• Whole blood is collected and processed in a closed, sterile tube
• During centrifugation, a gel barrier isolates the plasma from cellular components of the blood
• The BD Vacutainer PPT Tube is designed for the one-step preparation of undiluted plasma specimens for molecular diagnostic test methods or where undiluted plasma is required

Instructions For Use

• Prepare venipuncture site with an appropriate antiseptic. Use your institution’s recommended procedure for standard venipuncture technique and sample collection.
• Remove needle shield.
• Perform venipuncture.
• Place tube in holder and push tube forward until tube stopper has been penetrated.
• Release tourniquet as soon as blood appears in tube.
• Wait until tube has filled to its stated volume and blood flow ceases.
• Pull tube off needle inside holder.
• Remove tube from holder.
• After collection of whole blood in the BD PPT Tube, immediately and gently invert the BD PPT Tube 8–10 times.
• After mixing, the whole blood specimen may be stored up to six (6) hours at room temperature until centrifugation.
• Centrifuge BD PPT Tube in a balanced, swing-out rotor type centrifuge at room temperature at 1,100 RCF for a minimum of 10 minutes.

Note: Use of alternate centrifugation conditions may also provide acceptable performance; this should be evaluated and validated by the laboratory.

• To obtain an undiluted plasma sample, remove the BD Hemogard Closure (See Instructions for Removal of BD Hemogard Closure Section) and decant plasma into a separate vessel or aliquot plasma into a separate vessel using a transfer pipette.

NOTE: When using a transfer pipette be sure NOT to disturb the gel barrier with the tip of the pipette.