The TOTAL-FIX stool collection kit is a reliable, user-friendly single-vial system for collecting and preserving stool specimens. Designed for use by untrained personnel, it ensures accurate preservation of specimens for laboratory testing, enabling detection of various parasites, including helminth larvae and eggs, protozoan trophozoites and cysts, coccidian oocysts, and microsporidian spores.

Features:

• Single-Vial System: Simplifies stool collection and preservation for untrained users.
• Mercury, Formalin, and PVA-Free FIXative: Safer and eco-friendly solution for laboratories.
• Wide Applicability: Supports concentrations, stains, immunoassays, and molecular assays.
• Preserves Parasite Morphology: Ensures accurate analysis of helminths, protozoans, and spores.
• Multi-Language Instructions: Ten-language guide aids proper use by diverse users.
• Compatible with Advanced Testing: Maintains DNA/RNA integrity for pathogen molecular assays.
• Laboratory-Friendly: Resolves disposal and monitoring issues with conventional preservatives.

Intended Use:

The Total-Fix stool collection kit is a single vial system that provides a standardized method for untrained personnel to properly collect and preserve stool specimens for the detection of helminth larvae and eggs, protozoan trophozoites and cysts, coccidian oocysts, and microsporidian spores. Permanent stains, concentrations and most fecal immunoassays can be performed from a Total-Fix preserved specimen. Total-Fix is also an excellent DNA and RNA preservative for stool pathogen molecular assays. A ten language instruction sheet is provided to assist patients or healthcare professionals with the proper use of the kits at home or in the hospital.

Precautions:

1. Ethyl acetate and diethyl ether are flammable. Use in a well ventilated area. Keep away from direct flame. Avoid contact of the solution with skin and eyes. Should contact occur flush with running water. Avoid breathing fumes.
2. Avoid contact of Total-Fix solution with skin or eyes. If contact occurs, flush effected area with water. If irritation develops contact a physician immediately.
3. Total-Fix solution is poisonous. If ingestion occurs drink milk or water. Contact a physician immediately.
4. Every sample should be treated as a potential source of infection. Good laboratory practice should be followed at all times. The use of gloves and hand washing is recommended.

• The product warranty is applicable as per the terms and conditions provided by the product manufacturer.

Please call us for specific details.

• No returns will be accepted after 30 days from the date of shipment.
• All returns are subject to a restocking fee as per manufacturers terms and conditions.
• All returns must have an RGA number (Returned Goods Authorization), unauthorized returns will not be accepted.
• We do not guarantee fulfillment of any desired purpose or product suitability to the user and this will not be considered as a valid reason for return.
• The products must be new, unused condition, not tampered with, in original packaging and returned at the customers expense in the original packaging.
• If your return is not due to any manufacturing defect then the original shipping cost will be deducted from the total refund.
• Hygiene, bath and toilet items cannot be returned once opened or used.
• Standard manufacturer terms and conditions apply for return policy of this product.

Please call us for specific details.

Q1. What is TOTAL- FIX ?

A. TOTAL- FIX is a FIXative with a proprietary formula, it is similar to UniFIX and Zinc PVA (Z- PVA ), a commonly used fixative that has been commercially available and used in many laboratories since 1992.

Q2. What is PVA ?

A. PVA stands for polyvinyl alcohol, a plastic powder/resin that is incorporated into the fixative (Schaudinn’s or other fixatives such as Z- PVA ) and serves as an adhesive to “glue” the stool material onto the slide. PVA itself has no preservation capability.

Q3. Since PVA is used to “glue” the stool onto the slide prior to permanent staining, how does the material stick to the glass slide?

A. The TOTAL- FIX user can add some albumin to the slide prior to preparation of the stool smear for staining. However, if the stool smear is thoroughly dry prior to staining, albumin will not be necessary.

Q4 How long should the slides prepared from TOTAL- FIX be dried prior to processing for routine staining (trichrome or iron-hematoxylin)?

A. Spread the sample over the slide to prepare a thin smear which varies in thickness (routine preparation of slides – no changes required). Allow to dry overnight at room temperature or for several hours (minimum of 30 min; 60 min if slide is thicker) in a 37oC incubator or slide warmer (the smear will appear opaque when dry). Many labs routinely prepare their smears and then place the smears on a tray in the incubator (37ºC) for 60 min to dry. The fecal smears can be left longer in the incubator with no damage at all to the slides. Do not use a heating block; the higher temperature for most heating blocks will be detrimental to any organisms (protozoa, coccidia, microsporidia) present.

Q5. What should Giardia lamblia and other protozoa look like when stained from normal patient clinical specimens (no lag time between specimen passage and fixation)?

A. Organism morphology should appear no different from organisms preserved in Z- PVA , with good overall morphology for trophozoites, cysts (routine trichrome or iron-hematoxylin), coccidian oocysts (modified acid-fast stains), and microsporidial spores (modified Trichrome).